Elaboration of Pyrimidine-specific Nucleosidases by Human Lymphoblastoid Cells of Established Cultures1
نویسنده
چکیده
pyrimidine-specific nucleosidases which degraded radiola beled precursors used for measuring DNA and RNA synthe sis. Results of studies reported herein, and findings of others, demonstrate that CLC as well as peripheral blood leuko cytes of healthy subjects and patients with uncontrolled lymphocytic or myelocytic leukmeia release nucleosidases, and possibly other enzymes, when these cells are main tamed for a short time as a dense population. Failure to account for the possible presence of such enzymes in cul ture supernatants or cell homogenates may lead to the erroneous interpretation of data obtained in studies at tempting to characterize certain thermolabile lymphokines or chalones.
منابع مشابه
Elaboration of pyrimidine-specific nucleosidases by human lymphoblastoid cells of established cultures.
Pyrimidine-specific nucleosidases were released rapidly by human lymphoblastoid cells of established cultures when incubated under certain culture conditions having no adverse affect on their viability or morphology. Nucleosidase production was not restricted to any particular type of lymphoblastoid line; enzymes with a high level of activity were elaborated by cells of cultures initiated from ...
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